- ￥1350 - 3000
- Proteintech
- 13074-2-AP
- 2025年10月13日
- IHC, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CCK protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
IHC, ELISA
- 靶点：
CCK
- 抗体英文名：
CCK Polyclonal antibody
- 抗体名：
CCK Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive IHC detected in | human pancreas tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13074-2-AP targets CCK in IHC, IF, ELISA, Cell treatment applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA |
| 文献引用应用 | IF, Cell treatment |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag3725 Product name: Recombinant human CCK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-115 aa of BC008283 Sequence: MNSGVCLCVLMAVLAAGALTQPVPPADPAGSGLQRAEEAPRRQLRVSQRTDGESRAHLGALLARYIQQARKAPSGRMSIVKNLQNLDPSHRISDRDYMGWMDFGRRSAEEYEYPS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cholecystokinin |
| 别名 | CCK, CCK12 Cholecystokinin 8, CCK18 Cholecystokinin 12, CCK25 Cholecystokinin 18, CCK33 Cholecystokinin 25, CCK39 Cholecystokinin 33, CCK5, CCK7 Cholecystokinin 5, CCK8 Cholecystokinin 7, cholecystokinin |
| 计算分子量 | 115 aa, 13 kDa |
| GenBank蛋白编号 | BC008283 |
| 基因名称 | CCK |
| Gene ID (NCBI) | 885 |
| RRID | AB_2228728 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P06307 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cholecystokinin (CCK) is a classic gut hormone. CCK is also a complex system of peptides expressed in several molecular forms in enteroendocrine I cells, in cerebral and peripheral neurons, in cardiac myocytes and spermatozoa. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the peptide hormones cholecystokinin-8, -12, -33, and others. The encoded peptides have been shown to regulate gastric acid secretion and food intake.
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文献和实验| Species | Application | Title |
|---|---|---|
Neuron Molecular pathways and diagnosis in spatially resolved Alzheimer's hippocampal atlasAuthors - Pan Wang | ||
| Cell treatment | J Fungi (Basel) Two Cladosporium Fungi with Opposite Functions to the Chinese White Wax Scale Insect Have Different Genome Characters.Authors - Wei Liu | |
J Ethnopharmacol Tibetan medicine Ru-yi-Zhen-bao Pills exhibits anti-migraine effect through mediating PAG anti-nociceptive channel.Authors - Ya Min Luo | ||
| IF | Sci Total Environ Micro/nanoplastics impair the feeding of goldfish by disrupting the complicated peripheral and central regulation of appetiteAuthors - Weixia Zhang |
流式细胞仪、免疫磁珠及亲和板结合分离法分选c kit+肝癌细胞的比较
结合分离法分选肿瘤细胞：按文献中亲和板结合分离法，以兔抗人c kit多克隆抗体为一抗，FITC标记的羊抗兔IgG为二抗，分选c kit-细胞和c kit+细胞。每种方法分选出5组细胞，进行如下检测。 (1)细胞活力检测：采用台盼蓝排斥实验检测细胞活力并计算细胞存活率=［活细胞总数/（活细胞总数+死细胞总数）］×100%。 (2)细胞增殖能力试验：参照文献的CCK 8法检测并计算细胞刺激指数（stimulate Index，SI），SI=（实验孔OD值-空白孔OD值）/（阴性对照孔OD值-空白孔OD
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