- ￥1210 - 3020
- Proteintech
- CR-65251
- 2026年05月27日
- FC
- Mouse
- human
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- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Cardinal Red™ Fluorescent Dye
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
CD155/PVR
- 抗体英文名：
Cardinal Red™ Anti-Human CD155/PVR (SKII.4)
- 抗体名：
Cardinal Red™ Anti-Human CD155/PVR (SKII.4)
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥1210.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥3020.0 |
经过测试的应用
| Positive FC detected in | U-937 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CR-65251 targets CD155/PVR in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | poliovirus receptor |
| 别名 | PVR, CD155, HVED, Necl 5, NECL5 |
| 计算分子量 | 45 kDa |
| GenBank蛋白编号 | BC015542 |
| 基因名称 | CD155/PVR |
| Gene ID (NCBI) | 5817 |
| 偶联类型 | Cardinal Red™ Fluorescent Dye |
| 最大激发/发射波长 | 592 nm / 611 nm |
| 形式 | Liquid |
| 纯化方式 | N/A |
| UNIPROT ID | P15151 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.
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文献和实验的固有成分，如C1-C9、B、D、P因子及MBL相关蛋白，分别参与经典、旁路或凝集素途径，其中C5-C9为共同终末通路分子；二是补体调节蛋白，以可溶或膜结合形式控制活化程度，如C1INH、H因子、DAF、CD59等；三是补体受体，包括CR1-CR5、C3aR、C5aR等，介导补体片段的生物学效应。 补体激活途径 补体系统可通过三条主要途径激活：经典途径、旁路途径和凝集素途径。经典途径由抗原-抗体复合物触发，C1q识别并结合免疫球蛋白（IgM或IgG）的Fc段，在Ca²⁺依赖下激活C1r和C1
) or VE-8 (F) and the VP64 activator domain, stained with anti-CD144 and analyzed by FACS. Red, cells infected with the pMX vector containing the same DNA-binding domain but linked to the KRAB repression domain (SKD), and stained with anti-CD144 (anti-VE
ELISPOT (Enzyme-linked ImmunoSPOT)
Kreher CR, et al. 2003. CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J. Immunol. Methods. 278: 79-93. Ott PA, et al. 2004. CD28 costimulation enhances the sensitivity of the ELISPOT assay
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