- ￥1350 - 3000
- Proteintech
- 11278-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TOMM22 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
TOM22
- 抗体英文名：
TOM22 Polyclonal antibody
- 抗体名：
TOM22 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa, K-562 cells, HeLa cells, mouse liver tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11278-1-AP targets TOM22 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1797 Product name: Recombinant human TOMM22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC009363 Sequence: MAAAVAAAGAGEPQSPDELLPKGDAEKPEEELEEDDDEELDETLSERLWGLTEMFPERVRSAAGATFDLSLFVAQKMYRFSRAALWIGTTSFMILVLPVVFETEKLQMEQQQQLQQRQILLGPNTGLSGGMPGALPSLPGKI |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | translocase of outer mitochondrial membrane 22 homolog (yeast) |
| 别名 | TOMM22, 1C9 2, hTom22, MST065, MSTP065 |
| 计算分子量 | 15 kDa |
| 观测分子量 | 15-22 kDa |
| GenBank蛋白编号 | BC009363 |
| 基因名称 | TOMM22/Tom22 |
| Gene ID (NCBI) | 56993 |
| RRID | AB_2207542 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NS69 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises the 22 kDa protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Cell Res Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis.Authors - Bo Zhou |
| human | WB,IF | Cell Discov Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis.Authors - Fan Yu |
| human | WB | J Genet Genomics HPDL deficiency causes a neuromuscular disease by impairing the mitochondrial respiration.Authors - Yu Sun |
| human | WB | Mol Biol Cell Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.Authors - Shan Xu |
| human | WB | J Hum Genet Mutations in TOMM70 lead to multi-OXPHOS deficiencies and cause severe anemia, lactic acidosis, and developmental delay.Authors - Xiujuan Wei |
| human | WB | EMBO Rep UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagyAuthors - Jing Zheng |
Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
coupled with mass spectrometry (AP?MS) is a robust technique used to identify protein?protein interactions. With recent improvements in sample preparation, and dramatic advances in MS instrumentation speed and sensitivity, this technique
Rapid Inactivation of Proteins by Knocksideways
Figure 15.20.2 Other Mitotrap‐like constructs. HeLa cells expressing an FKBP‐tagged AP‐2 subunit were transiently transfected with either an HA‐tagged construct consisting of the Tom70 mitochondrial outer
3, Pages 291-298 freecell 另外，这篇文章想必你也看过了。可以追踪一下作者的文章或者引用此文的文献。 Sci. STKE, 22 May 2007 Vol. 2007, Issue 387, p. pe25 Micromanagement During the Innate Immune Response Innate immunity, a well-studied
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