- ￥1350 - 3000
- Proteintech
- 10436-1-AP
- 2025年10月13日
- WB, IHC, IP, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Caspase 2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IP, ELISA
- 靶点：
Caspase 2/P32/P18
- 抗体英文名：
Caspase 2/P32/P18 Polyclonal antibody
- 抗体名：
Caspase 2/P32/P18 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Staurosporine treated Jurkat cells, HeLa cells, mouse liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10436-1-AP targets Caspase 2/P32/P18 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA |
| 文献引用应用 | WB, IF, RIP, ELISA |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rabbit |
| 免疫原 |
CatNo: Ag0694 Product name: Recombinant human Caspase 2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 18-217 aa of BC002427 Sequence: MAADRGRRILGVCGMHPHHQETLKKNRVVLAKQLLLSELLEHLLEKDIITLEMRELIQAKVGSFSQNVELLNLLPKRGPQAFDAFCEALRETKQGHLEDMLLTTLSGLQHVLPPLSCDYDLSLPFPVCESCPLYKKLRLSTDTVEHSLDNKDGPLCLQVKPCTPEFYQTHFQLAYRLQSRPRGLALVLSNVHFTGEKELE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | caspase 2, apoptosis-related cysteine peptidase |
| 别名 | CASP2, Caspase 2, CASP 2, CASP-2, Caspase 2/p18 |
| 计算分子量 | 452 aa, 51 kDa |
| 观测分子量 | 48-51 kDa, 32 kDa, 18kDa |
| GenBank蛋白编号 | BC002427 |
| 基因名称 | Caspase 2 |
| Gene ID (NCBI) | 835 |
| RRID | AB_2228450 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42575 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | IF | Nat Cell Biol Caspase-2 is a condensate-mediated deubiquitinase in protein quality controlAuthors - Yingwei Ge |
| human | WB, IF | Front Microbiol Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.Authors - Bei Wang |
Front Pharmacol PMID: 32210806Authors - Wen Su | ||
| rabbit | RIP | Life Sci Timolol induces necroptosis, apoptosis and senescence concentration-dependently in rabbit Limbal stem cells in vitro.Authors - Guo-Jian Jiang |
| human | ELISA | Toxicol In Vitro PMID: 32320760Authors - Ting Yang |
| mouse | WB | Toxicon Involvement of caspase in patulin-induced hepatotoxicity in vitro and in vivo.Authors - Baigang Zhang |
的Caspase-3及对底物多聚（ADP-核糖）聚合酶[poly（ADP-ribose）polymerase,PARP]等的裂解。方法：收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭，室温1.5~2h或4°C过夜→Caspase-3多抗或单抗室温反应1~2h或4°C过夜→TBS-T（含0.05% Tween 20的TBS）洗3次，5~10min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS-T洗3次
的Caspase-3及对底物多聚（ADP-核糖）聚合酶[poly（ADP-ribose）polymerase，PARP]等的裂解。方法： 收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭，室温1.5~2h或4°C过夜→Caspase-3多抗或单抗室温反应1~2h或4°C过夜→TBS-T（含0.05% Tween 20的TBS）洗3次，5~10min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS-T洗3次
阶段有关。ICH-1L和ICH-1S均表达于心脏、肾和脑，但ICH-1S在胚脑中表达最高，而ICH-1H在成年胸腺中表达最高。 在细胞发生凋亡时，48kDa的caspase-2首先被一种caspase-3样蛋白酶在Asp316剪切成p33和p14，然后再缓慢地在Asp152和Asp330处剪切为p18和p12组成的活性蛋白酶，后一过程要在2~3h后才能完成。caspase-2可能参与CTL细胞的杀伤作用，但它并不能被颗粒酶B直接活化，必须被一种caspase-3样蛋白
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