- ￥1350 - 3000
- Proteintech
- 10121-2-AP
- 2025年10月13日
- WB, IHC, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human ICAM2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
ICAM2/CD102
- 抗体英文名：
ICAM2/CD102 Polyclonal antibody
- 抗体名：
ICAM2/CD102 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, RAW 264.7 cells, mouse brain tissue, HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue, mouse spleen tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10121-2-AP targets ICAM2/CD102 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0165 Product name: Recombinant human ICAM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 103-275 aa of BC003097 Sequence: NSNVSVYQPPRQVILTLQPTLVAVGKSFTIECRVPTVEPLDSLTLFLFRGNETLHYETFGKAAPAPQEATATFNSTADREDGHRNFSCLAVLDLMSRGGNIFHKHSAPKMLEIYEPVSDSQMVIIVTVVSVLLSLFVTSVLLCFIFGQHLRQQRMGTYGVRAAWRRLPQAFRP |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | intercellular adhesion molecule 2 |
| 别名 | ICAM2, CD102, ICAM-2, ICAM 2 |
| 计算分子量 | 31 kDa |
| 观测分子量 | 55-80 kDa |
| GenBank蛋白编号 | BC003097 |
| 基因名称 | ICAM2/CD102 |
| Gene ID (NCBI) | 3384 |
| RRID | AB_2233415 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13598 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Front Cell Infect Microbiol Brain Microvascular Endothelial Cell-Derived HMGB1 Facilitates Monocyte Adhesion and Transmigration to Promote JEV Neuroinvasion.Authors - Song-Song Zou |
| human | WB,IHC | PLoS One ICAM-2 expression mediates a membrane-actin link, confers a nonmetastatic phenotype and reflects favorable tumor stage or histology in neuroblastoma.Authors - Yoon Karina Jin KJ
|
| human | IHC | J Ovarian Res The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomasAuthors - Xinxin Xu |
| human | IHC | Hum Pathol Intercellular adhesion molecule 1/2 and E-selectin in plasma cell mastitis: immunohistochemical study of 35 cases.Authors - Yu Dong |
| human | IF | Stem Cell Res Ther MSCs promote the efferocytosis of large peritoneal macrophages to eliminate ferroptotic monocytes/macrophages in the injured endometriaAuthors - Jiali Wang |
| human | WB,IHC,IF | Lupus Sci Med Proteomics uncovers ICAM2 (CD102) as a novel serum biomarker of proliferative lupus nephritisAuthors - Zhengyong Li
|
，取决于你的喜好和方便。1ml不好装，除非有专门的小柱子，对于直径和高度没有特别的要求，我们的做法是： 1、 GST琼脂糖凝胶FF装柱，0.7X2.5cm，柱床体积为1ml； 2、 用缓冲液1平衡5个床体积，流速为1ml/min； 3、 将10ml发酵液用缓冲液1稀释到20ml，0.45μm滤膜过滤，上样。流速为1ml/min； 4、 用缓冲液1再洗10个床体积，流速为1ml/min； 5、 用缓冲液2洗脱，流速为1ml/min，收集洗脱峰 6、 用纯水洗5个柱床体积，再用20
商品名称。所以你理解是有偏差的，此外agarose不一定是琼脂糖凝胶，而Glutathione-agarose（4%cross-linked beaded agarose）和Glutathione Sepharose 4B是同一类东西，只是厂家不同。 GST融合蛋白纯化过，5ml的凝胶你可以装在1.6x20的柱子上用，也可以按照分子克隆的做法做，取决于你的喜好和方便。1ml不好装，除非有专门的小柱子，对于直径和高度没有特别的要求，我们的做法是： 1、GST琼脂糖凝胶FF装柱，0.7X2.5cm
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