- ￥1350 - 3000
- Proteintech
- 16659-1-AP
- 2025年10月09日
- WB, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human DNASE1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
DNase I
- 抗体英文名：
DNase I Polyclonal antibody
- 抗体名：
DNase I Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | human urine sample |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16659-1-AP targets DNase I in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag10117 Product name: Recombinant human DNASE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-282 aa of BC029437 Sequence: MRGMKLLGALLALAALLQGAVSLKIAAFNIQTFGETKMSNATLVSYIVQILSRYDIALVQEVRDSHLTAVGKLLDNLNQDAPDTYHYVVSEPLGRNSYKERYLFVYRPDQVSAVDSYYYDDGCEPCGNDTFNREPAIVRFFSRFTEVREFAIVPLHAAPGDAVAEIDALYDVYLDVQEKWGLEDVMLMGDFNAGCSYVRPSQWSSIRLWTSPTFQWLIPDSADTTATPTHCAYDRIVVAGMLLRGAVVPDSALPFNFQAAYGLSDQLAQAISDHYPVEVMLK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | deoxyribonuclease I |
| 别名 | DNASE1, EC:3.1.21.1, DRNI, DNL1, Deoxyribonuclease-1 |
| 计算分子量 | 282 aa, 31 kDa |
| 观测分子量 | 31-40 kDa |
| GenBank蛋白编号 | BC029437 |
| 基因名称 | DNASE1 |
| Gene ID (NCBI) | 1773 |
| RRID | AB_3669253 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P24855 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Deoxyribonuclease I (DNase I), also known as DNL1 and DRNI, belongs to the DNase I family. DNase I is a DNA-digestive glycoprotein that cleaves both single- and double stranded DNA through hydrolysis of the phosphodiester bond. In humans, the DNase I enzyme is primarily secreted by the pancreas, parotid, and pituitary glands into the digestive system and bloodstream. Since DNase I is ubiquitously expressed in human tissues, it is thought to play a similar anti-autoimmune role in other organs as well (PMID: 21806320). The calculated molecular weight of DNase I is 31 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which is caused by glycosylation (PMID: 34329318).
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文献和实验（Promega）；Genomyx LR 、 Genomyx SC、DNAThermal Cycler（Perkin Elmer）1.3 总RNA的制备按试剂盒提供的方法分别提取三种细胞的总 RNA,以 RNase-free DNase I（终浓度80 000 U/L）除去其中污染的 DNA,经甲醛变性凝胶电冰鉴定其完整性，并以紫外分光光度计检 测其纯度1.4 mRNA差异显示1.4.1 逆转录反应选择锚定引物[T7（dT12）AP(anchored prime rs，AP)]，序
96-well RNA In Situ Hybridization Protocol
directly. 2. Using a multichannel pipettor, add 5ul of 2X polymerase mix. 3. Incubate at 37 degrees C for 2 hrs. 4. Add 10ul DNase I mix. 5. Incubate at 37 degrees C for 15 min. 6. Using multichannel pipettor, add 80ul 125 mM NaCO
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling
control slide: in DNase I solution (100µl of 200µg/ml), 10 min., RT. 1x PBS rinse, 2 times in a separate container then combine with other slides.)Wipe around tissue.Make up negative Control solution (just Label solution containing FITC) and TUNEL
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