- ￥1350 - 3000
- Proteintech
- 10782-2-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ChIP, ELISA
- Rabbit
- human, mouse, rat, zebrafish
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, zebrafish
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ChIP, ELISA
- 靶点：
TDP-43
- 抗体英文名：
TDP-43 Polyclonal antibody
- 抗体名：
TDP-43 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, HeLa cells, C2C12 cells, Neuro-2a cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue, human brain tissue, human brain (FTLD-U) tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive ChIP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:3000-1:12000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Chromatin immunoprecipitation (ChIP) | CHIP : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10782-2-AP targets TDP-43 in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, IEM applications and shows reactivity with human, mouse, rat, zebrafish samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ChIP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA, IEM |
| 经测试反应性 | human, mouse, rat, zebrafish |
| 文献引用反应性 | human, mouse, rat, monkey, chicken, zebrafish, hamster, yeast, horse, seal |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TAR DNA binding protein |
| 别名 | TDP-43 (N-terminal), TARDBP, TDP 43, TDP43, ALS10 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | BC001487 |
| 基因名称 | TDP-43 |
| Gene ID (NCBI) | 23435 |
| RRID | AB_615042 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13148 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Nature Oxidative cyclization reagents reveal tryptophan cation-π interactionsAuthors - Xiao Xie |
| mouse | IF | Cell Mouse oocytes sequester aggregated proteins in degradative super-organellesAuthors - Gabriele Zaffagnini |
| human | WB | Lancet Neurol A C9orf72 promoter repeat expansion in a Flanders-Belgian cohort with disorders of the frontotemporal lobar degeneration-amyotrophic lateral sclerosis spectrum: a gene identification study.Authors - Ilse Gijselinck |
| mouse | IF | Cell Res Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologiesAuthors - Liang Guo |
| human | WB | Nature MIR-NATs repress MAPT translation and aid proteostasis in neurodegeneration.Authors - Roberto Simone |
| human | IF | Nature TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A.Authors - X Rosa Ma |
的沉淀线即可以肉眼观察也可利用染色方法观察。 实验试剂 1. 纯化的抗体；蛋白质混合物；1%琼脂。 2. Tris-巴比妥缓冲溶液：2.24g 巴比妥酸，4.43g Tris，0.053g 乳酸钙及0.065g 叠氮化钠溶于100ml 蒸馏水中。 3. 电泳缓冲溶液：将Tris-巴比妥缓冲溶液与蒸馏水按1：4的比例混合。 实验设备 水平电泳仪；打孔器；滴管；刀片；电源；水浴(55℃
妥酸，4.43g Tris，0.053g 乳酸钙及0.065g 叠氮化钠溶于100ml 蒸馏水中。 ⑶ 电泳缓冲溶液：将Tris-巴比妥缓冲溶液与蒸馏水按1：4的比例混合。 【实验方法】 ⒈ 准备琼脂糖凝胶 将琼脂和电泳缓冲溶液混合放入90℃水浴加热至溶化，制成1%琼脂，将琼脂在冷却前铺在水平玻璃板上，待冷却至室温后，按图用内径4毫米的打孔器打孔及制作凹槽。 ⒉ 电泳 将用电泳缓冲溶液稀释的4%-5%的抗原用滴管小心放入小孔中，将胶板放入电泳槽中并用润湿的滤纸将胶板和电泳槽中的缓冲
枪(5-100μl)。 ⒉ 实验试剂 ⑴ 纯化的抗体;蛋白质混合物;1%琼脂。 ⑵ Tris-巴比妥缓冲溶液：2.24g 巴比妥酸，4.43g Tris，0.053g 乳酸钙及0.065g 叠氮化钠溶于100ml 蒸馏水中。 ⑶ 电泳缓冲溶液：将Tris-巴比妥缓冲溶液与蒸馏水按1：4的比例混合。 【实验方法】 ⒈ 准备琼脂糖凝胶 将琼脂和电泳缓冲溶液混合放入90℃水浴加热至溶化，制成1%琼脂，将琼脂在冷却前铺在水平
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