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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
室温,避光,12个月
- 库存:
100
- 供应商:
信帆生物
产品名称:TCA(10%w/v)
简单介绍:多种用途
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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
SDS Gel Electrophoresis of Tubulin\MAPs
2% SDS 10% glycerol 5% -mercaptoethanol 0.001 % bromophenol blue TEMED 10% (w/v) Ammonium persulphate 50% and 7% (w/v) TCA (trichloroacetic acid) 0.1% (w/v) Coomassie brilliant blue in 50
In Vitro Transcription Assay (Run-off Assay) using Permeabil
in 3 to 7 days. F. TCA Precipitation to Determine Incorporation of [32 P] GTP into Nucleic Acid (see Hint #8) 1. Add 1 μl aliquot of reaction assay to a 13 x 100 mm tube containing 2 ml of Yeast tRNA Solution. 2. Add 1 ml of 10% TCA. Mix. 3. Incubate on ice
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