- ¥6370
- LONZA
- PT-3003
- 2025年08月14日
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- 技术资料
- 英文名：
hMSC Chondrogenic Differentiation BulletKit™ Medium
- 规格：
kit
hMSC Chondrogenic Differentiation BulletKit™ Medium
品牌：Lonza 货号：PT-3003
属性：hMSC分化培养基
Shelf Life(month)：12
产地：USA
存储条件：2-8
A serum containing medium designed to induce chondrogenic differentiation, with the addition of TGF-β3, of human bone marrow derived mesenchymal stem cells into mature, functionally active chondrocytes in as few as 14 days. Differentiation can then be validated by a variety of methods including staining with alcian blue or safranin.
The hMSC Chondrogenic Differentiation BulletKit™ Medium, with the addition of TGF-β3 (sold separately) provides a differentiation medium guaranteed to induce chondrogenic differentiation of human bone marrow derived mesenchymal stem cells into mature, functionally active chondrocytes; Each bottle of the hMSC Chondrogenic Differentiation BulletKit™ Medium contains enough differentiation medium to fully differentiate at least 2 million human bone marrow derived mesenchymal stem cells [approximately 4 complete 24-well plates] into mature, functionally active chondrocytes.
The hMSC Chondrogenic Differentiation Medium is offered as a BulletKit™ Medium (catalog no. PT-3003) which includes both the basal media and the necessary supplements (with the addition of TGF-β3) for chondrogenic differentiation of human bone marrow derived mesenchymal stem cells. The basal medium (Differentiation Basal Medium - Chondrogenic; catalog no. PT-3925) and the necessary supplements (HMSC SQ Kit - Chondro; catalog no. PT-4121 and TGF Beta 3 for HMSC Chondro diff; catalog no. PT-4124) can also be purchased individually. (PT-3925 & PT-4121)
Partial List of Cells Differentiated with hMSC Chondrogenic Differentiation BulletKit™ Medium*:
Human bone marrow derived stem cells
Human umbilical cord blood derived stem cells
Human umbilical cord matrix derived stem cells
C57BL/6 mice bone marrow derived stem cells
human adipose derived stem cells
包装：基础培养基：185ml；添加剂：ITS +supplement, dexamethasone, ascorbate, sodium pyruvate, proline, GA-1000, L-glutamine
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文献和实验R&D Systems:如何提高MSC的扩增且不损失其分化潜能
&DSystems）诱导hMSCs分化为骨细胞。将0.5 mL完全成骨基础培养基中7.4×103量级的hMSCs植入含有无菌盖玻片的24孔培养皿的每个孔中。37℃下，培养皿在含有5% CO2的增湿孵化器中孵育1至2天，直至50%至70%的细胞汇合，然后用0.5 mL的完全成骨分化培养基替换每个孔中的培养基。每3至4天更换一次分化培养基，14天至21天期间骨细胞可见。用免疫细胞化学技术鉴定分化的骨细胞。 成软骨分化。用S temX Vi v o 人/ 小鼠成软骨基础培养基（ 目录
%的密度（24-72小时）。3、诱导间充质干细胞将其中一份培养的细胞用MSC成骨分化培养基诱导，另一份培养的细胞用 MSC生长培养基培养作为阴性对照。4、间充质干细胞的分化培养诱导培养14天，每3天更换一次培养基。操作时候小心不要破坏细胞的单层。 要点：在整个染色过程中不要让细胞在干的状态下放置超过30秒！三、向成软骨分化的操作流程 1、接种间充质干细胞 在96 U型底板中用MSC生长培养基接种间充质干细胞，每接种1x105个MSC。 2、MSC芽体形成 在24-48小时
中都有着80%以上的转染效率。对于难转染的细胞，如Jurkat细胞，它比竞争产品的转染效率高出40倍。此外，Xfect毒性也较低。以往，由于转染试剂不能耐受血清，转染过程中还得换成无血清培养基，甚是麻烦。Xfect则不受血清的影响，转染全程都可以有血清的存在，使用更方便。Xfect的价格为2001元/100次，注意，这里的100次是以6孔板的孔计算的，如果换算成24孔板，大约则是500次。与大牌的转染试剂相比，性价比颇高。(价格仅供参考) Xfect mESC转染 试剂则是专为小鼠
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