荧光标记微管蛋白，Tubulin (HiLyte 488TM

• Fluorescent microtubule substrates for in vitro kinesin motility assays
• Microtubule dynamics studies in vitro
• Analyze effects of MAPs & drugs on microtubule dynamics in vitro

Tubulin protein T240 is labeled on random surface lysines using HiLyte™488 activated ester;

• Ex: 502 nm, Em:527 nm – green spectrum
• Labeling stoichiometry: ~1–2 dyes per tubulin heterodimer
• Supplied as a lyophilized powder

Protein purity is assessed by scanning densitometry of Coomassie Blue stained protein on a12% polyacrylamide gel. Purity is determined to be >99% pure.

TL488M tubulin retains high biological activity, with >80% polymerizing into microtubules in vitro, as confirmed by a spin-down assay. This performance is comparable to that of unmodified tubulin.

CAT. NO.     PRODUCT     AMOUNT
T333P-A     Tubulin (Biotin Conjugate; Porcine Brain)     5 x 20 µg
T333P-B     Tubulin (Biotin Conjugate; Porcine Brain)     20 x 20 µg
T333P-XL     Tubulin (Biotin Conjugate; Porcine Brain)     1 x 500 µg
TL440M-A     Tubulin (AMCA dye labeled; porcine, micro-injctn)     5 x 20 µg
TL440M-B     Tubulin (AMCA dye labeled; porcine, micro-injctn)     20 x 20 µg
TL488M-A     Tubulin (HiLyte 488TM dye labeled; porcine, micro-injctn)     5 x 20 µg
TL488M-B     Tubulin (HiLyte 488TM dye labeled; porcine, micro-injctn)     20 x 20 µg
TL590M-A     Tubulin (Rhodamine labeled; porcine, micro-injctn)     5 x 20 µg
TL590M-B     Tubulin (Rhodamine labeled; porcine, micro-injctn)     20 x 20 µg
TL620M-A     Tubulin (X-rhodamine labeled; Bovine Brain micr-injctn)     5 x 20 µg
TL620M-B     Tubulin (X-rhodamine labeled; Bovine Brain micr-injctn)     20 x 20 µg
TL670M-A     Tubulin (HiLyte 647TM dye labeled; porcine, micro-injctn)     5 x 20 µg
TL670M-B     Tubulin (HiLyte 647TM dye labeled; porcine, micro-injctn)     20 x 20 µg