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文献和实验MEDIA FOR EMBRYO CULTURE AND MANIPULATION
Ingredient % of Final Volume 1 x DMEM with 20mM HEPES buffer 80.8% Penicillin/Streptomycin (5,000 i.u/ml/5,000ug/ml) 1% L-glutamine (200mM, 100x) 1% non-essential amino acid (100x) 1% nucleosides (100x) 1% b-mercaptoethanol (0.1M) 0.2
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) can be added in conjunction with NBT to increase the reaction rate further. Prepare a 100X stock of 2 mg/ml in H2O and use immediately. PMS is very unstable. To aid in identification of stained cells, the tissue can be processed for cryostat sections
Purification of recombinant sBRF M166L
Imidazole H2O to 200 ml final Elution Buffer, pH 8.0 (50 ml) 20mM Tris 8.0 0.12 g Tris 300mM NaCl
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