Hamster IgG (purified) (1 gram

Expression of IgG Antibodies in Mammalian Cells

Production of IgG antibodies is usually done in mamalian cells. Predominantly, chinese hamster ovary (CHO) cells are used for generating stably transfected cell lines and for manufacturing. In addition to large scale manufacturing, stably

Capture of the Human IgG1 Antibodies by Protein A for the Kinetic Study of h-IgG/FcR Interaction Using SPR-Based Biosensor Technology

Surface plasmon resonance (SPR) is a key technology to evaluate IgG effector functions in vitro involving binding to Fcgamma receptors (FcγR). We describe here the chemical coupling of protein A to a sensor chip. IgGs prepared

Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

properties of IgGs, they can be expressed and purified in the same manner as regular antibodies. IgG–citrine fusions not only retain the binding properties (affinity and specificity) of antibodies but also contain Fc-regions (useful for immunoassay