Armenian Hamster IgG Isotype C

immunofluorescence staining

require compensation controls where the same cells are stained with each of the fluorochromes separately. The correct negative control for allimmuno fluorescent experiments are cells treated in the same way but incubated with isotype matched control

流式细胞基本知识问答

不同，应选用相同来源的未标记单抗作为同型对照来调整背景染色。举个例子：比如检测一抗为单抗的mouse anti rat CD11b，clone OX-42 purified IgG，那么它的isotype 是mouse IgG2a， 所以可以用purified（纯化的） mouse IgG2a来做OX-42的同型对照（Isotype Control）。一般的的生物技术公司和国内的代理都有出售。 同型对照：是指与MoAb相同的、未免疫小鼠的免疫球蛋白亚类，若使用直接

（共享）蛋白分析方法之免疫沉淀

by a non-relevant immunoglobulin (i.e, normal serum for polyclonal antibodies, control mouse ascites fluid for ascites, and isotype controls for purified mouse monoclonal antibodies). 3) Proteolytic digestion can occur when cells are lysed and contents