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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
UAGPase | UDP-GlcNAc pyrophosphorylase
- 规格:
50ul
| Immunogen |
| His-tagged full length recombinant UAGPase of Arabidopsis thaliana, overexpressed and purified from E.coli, UniProt:O64765,, TAIR:AT2G35020. Sequence used for immunization is conserved in both isoforms: UDP-N-acetylglucosamine diphosphorylase 1 (Q940S3) and (O64765) |
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Host Rabbit
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Clonality Polyclonal
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Purity Serum
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Format Lyophilized
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Quantity 50 µl
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Reconstitution For reconstitution add 50 µl of sterile water.
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Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Tested applications Western blot (WB)
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Recommended dilution 1 : 10 000 (WB)
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Expected | apparent MW
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| 55.76 kDa |
名称:UAGPase | UDP-GlcNAc pyrophosphorylase
货号:AS14 2829
品牌:Agrisera
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文献和实验To characterize and purify glycosyltransferases, it is essential to establish a simple and sensitive assay method. Here, we describe a method for determination of the activity of GnT VI (UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc
. We also describe the measurement of OGT activity by using synthetic peptides as acceptors and radiolabeled UDP-GlcNAc. Finally, a sensitive HPAEC-based technique to measure the cellular content of UDP-GlcNAc, the donor substrate of OGT, is described in detail.
Cancer Cell:曹雪涛院士团队揭示肿瘤相关巨噬细胞中葡萄糖代谢的增加可促进癌症转移
导读 代谢重编程广泛参与宿主与肿瘤之间的相互作用,并可决定肿瘤的进展和预后。长期以来,肿瘤细胞一直被认为是肿瘤微环境(TME)中葡萄糖的主要消耗者,在有氧糖酵解过程中产生乳酸。其中,髓系细胞,特别是肿瘤相关巨噬细胞(TAMs),在 TME 中消耗最多的葡萄糖,这表明巨噬细胞代谢需要更多葡萄糖的利用。 己糖胺生物合成途径(HBP)是糖代谢的主要途径,可合成核苷酸糖 UDP-GlcNAc,随后用于调节营养感知和应激反应的细胞内蛋白质的翻译后修饰——O-GlcNAc 糖基化修饰(O
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