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- Biolegend
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- 2025年11月14日
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上海觅拓生物
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文献和实验Intracellular Immunofluorescent Staining for Flow Cytometry
Wash in cold Flow Cytometry Staining Buffer (or cold PBS). Resuspend cell pellet with pulse vortex and add 1 ml of freshly prepared Fixation/Permeabilization working solution to each sample. Pulse vortex again. Incubate at 4°C for 30 - 60
Intracellular Cytokine Staining Protocol
A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell level. In this protocol, cells
Intracellular Staining Protocol
with minor loss in signal strength. 5. Wash cells twice in staining media (PBS containing 1% BSA) then resuspend in staining media at .5-1x106 cells per 100ul. 6. Stain cells - Add optimal concentrations of antibody and incubate for 15-30 min at RT
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