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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉恒意赛生物
- 检测范围:
156.25-10000 pg/mL
- 适应物种:
人
- 样本:
serum, plasma, tissue homogenates, cell lysates, saliva, cell culture supernates and other biological fluids
- 规格:
96T
| 中文名称 | 英文名称 | 规格 | 保存条件 |
| 抗体预备酶标板(可拆) | Pre-coated Microplate | 8×12 | 4°C |
| 冻干标准品 | Standard (lyophilized) | 2支 | 4°C |
| 标准品&样品通用稀释液 | Standard Diluent Buffer | 20mL | 4°C |
| 生物素结合物(100×) | Biotinylated-Conjugate (100x) | 60μL | 4°C |
| 生物素结合物稀释液 | Biotinylated Conjugate Diluent | 10mL | 4°C |
| 链霉素-辣根过氧化物酶(100×) | Streptavidin-HRP(100×) | 120μL | 4°C |
| 酶稀释液 | HRP Diluent | 12mL | 4°C |
| 浓缩洗涤液(25×) | Wash Buffer (25×) | 20mL | 4°C |
| 显色底物溶液(TMB) | TMB Substrate Solution | 9mL | 4°C(避光) |
| 反应终止液 | Stop reagent | 6mL | 4°C |
| 封板覆膜 | Plate Covers | 4张 | |
| 产品说明书 | Instruction manual | 1份 |
- 本试剂盒采用竞争法原理。用纯化的人 GHRL包被微孔板,制成固相载体,往包被有人 GHRL的微孔中依次加入标本或标准品、HRP 标记的抗人 GHRL特异性抗体,混匀后温育。经过彻底洗涤后用底物 TMB 显色。TMB 在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的人 GHRL呈负相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。
- 标准规格酶标仪(含波长450nm)
- 高速离心机
- 电热恒温箱
- 混匀器
- 高精度移液器,EP管及一次性吸头(0.5-10μL、2-20μL、20-200μL、200-1000μL)
- 双蒸水或去离子水,容量瓶,吸水纸等。
检测范围:156.25-10000 pg/mL
特异性和重复性
- 特异性:可检测重组或天然的人GHRL,且不与其它相关蛋白交叉反应
- 重复性:板内、板间变异系数均<10%
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