Anti-GAPDH Polyclonal Antibody

产品简介

单位	支
靶标	GAPDH
别名	G3PD;GAPD;HEL-S-162eP
宿主	Rabbit
稀释比例	WB 1:8000-12000. IHC 1:50-200.
Gene ID	2597
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	P04406
蛋白分子量	32/36kDa
储存液	Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
亚细胞定位	Cytoplasm Nucleus
免疫原	Recombinant protein of human GAPDH
亚型	IgG
纯化方法	Affinity purification
背景资料	Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Public Immunogen Range	1-336aa
克隆类型	Polyclonal Antibody
交叉反应	Human Rat Mouse
应用	WB IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图

Immunohistochemistry of paraffin-embedded Human breast cancer using GAPDH antibody diluted at 1:100

Western blot analysis with GAPDH antibody diluted at 1:8000;Lane:HEK293,HeLa,293T,HepG2,COS7,A431,Mouse liver,Mouse kidney,Rat liver