Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
亚细胞定位
Cytoplasm Nucleus
免疫原
Recombinant protein of human GAPDH
亚型
IgG
纯化方法
Affinity purification
背景资料
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Public Immunogen Range
1-336aa
克隆类型
Polyclonal Antibody
交叉反应
Human Rat Mouse
应用
WB IHC
规格
50ul 100ul
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示:
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图
Immunohistochemistry of paraffin-embedded Human breast cancer using GAPDH antibody diluted at 1:100
Western blot analysis with GAPDH antibody diluted at 1:8000;Lane:HEK293,HeLa,293T,HepG2,COS7,A431,Mouse liver,Mouse kidney,Rat liver