- ￥960 - 1600
- Solarbio
- 北京
- K001453M
- 2025年07月10日
- ICC ; IF ; WB
- Mouse
- Human Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic Peptide
- 亚型：
IgG1
- 形态：
液体
- 保存条件：
Store at -20°C. Avoid freeze / thaw cycles.
- 克隆性：
单克隆
- 标记物：
详询
- 适应物种：
Human Mouse
- 保质期：
详询
- 抗原来源：
详询
- 目录编号：
K001453M
- 级别：
详询
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Mouse
- 应用范围：
ICC ; IF ; WB
- 浓度：
详询
- 靶点：
Histone H2A.X(Phospho-Ser139)
- 抗体英文名：
Anti-Histone H2A.X(Phospho-Ser139) Monoclonal Antibody
- 抗体名：
H2A.X;H2A/X;H2AX
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥960.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1600.0 |
产品简介
|
英文名称
|
Anti-H2AFX(Phospho-Ser139) Monoclonal Antibody
|
|
中文名称
|
H2A组蛋白家族成员X抗体
|
|
靶标
|
H2AFX(Phospho-Ser139)
|
|
别名
|
H2AFX;H2A.X;H2A/X;H2AX;histone H2AX
|
|
背景资料
|
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
|
|
Swiss Prot
|
P16104
|
|
Gene ID
|
3014
|
|
亚细胞定位
|
Chromosome Nucleus
|
|
宿主
|
Mouse
|
|
克隆类型
|
Monoclonal Antibody
|
|
亚型
|
IgG1
|
|
克隆号
|
7G9-H4
|
|
交叉反应
|
Mouse ; Human
|
|
蛋白分子量
|
15kDa
|
|
应用
|
ICC ; IF ; WB
|
|
稀释比例
|
WB 1:500-1000. IF 1:50-200. ICC 1:50-200.
|
|
免疫原
|
A synthetic peptide of Human H2AFX(Phospho-Ser139)
|
|
纯化方法
|
Affinity purification
|
|
储存液
|
Buffer: PBS with 0.02% sodium azide, 0.5% BSA, 50% glycerol, pH7.3.
|
|
保存
|
Store at -20℃. Avoid freeze / thaw cycles.
|
|
规格
|
100ul ; 50ul
|
|
单位
|
支
|
These protocols are for reference only. Solarbio does not independently validate these methods.
Quick spin before use. If you have any questions, please contact us.
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
Monoclonal Antibody Production Protocol
of debris, rounded and refractive under phase contrast, and that they are harvested in log or late log phase growth (between 3.5 and 9 x105 cells/ml). Thaw cells 7 days before scheduled fusion. Myeloma do not grow well after being in culture
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
