- ￥960 - 1600
- Solarbio
- 北京
- K000096M
- 2025年07月15日
- IF ; ICC ; WB
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant human Smad2 protein fragments expressed in E.coli.
- 亚型：
IgG1
- 形态：
液体
- 保存条件：
Store at -20°C. Avoid freeze / thaw cycles.
- 克隆性：
单克隆
- 标记物：
详询
- 适应物种：
Human
- 保质期：
详询
- 抗原来源：
详询
- 目录编号：
K000096M
- 级别：
详询
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Mouse
- 应用范围：
IF ; ICC ; WB
- 浓度：
详询
- 靶点：
Smad2
- 抗体英文名：
Anti-Smad2 Monoclonal Antibody
- 抗体名：
hMAD-2;hSMAD2;JV18;JV18-1;MADH2;MADR2
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥960.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1600.0 |
产品简介
|
英文名称
|
Anti-Smad2 Monoclonal Antibody
|
|
靶标
|
Smad2
|
|
别名
|
hMAD-2;hSMAD2;JV18;JV18-1;MADH2;MADR2
|
|
背景资料
|
The protein encoded by this gene belongs to the SMAD,a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C.elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.This protein mediates the signal of the transforming growth factor (TGF)-beta,and thus regulates multiple cellular processes,such as cell proliferation,apoptosis,and differentiation.This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein.In response to TGF-beta signal,this protein is phosphorylated by the TGF-beta receptors.The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4.The association with SMAD4 is important for the translocation of this protein into the nucleus,where it binds to target promoters and forms a transcription repressor complex with other cofactors.This protein can also be phosphorylated by activin type 1 receptor kinase,and mediates the signal from the activin.Alternatively spliced transcript variants have been obs*erved for this gene.[provided by RefSeq,May 2012]
|
|
Swiss Prot
|
Q15796
|
|
Gene ID
|
4087
|
|
亚细胞定位
|
Cytoplasm Nucleus
|
|
宿主
|
Mouse
|
|
克隆类型
|
Monoclonal Antibody
|
|
亚型
|
IgG1
|
|
克隆号
|
6H5-E3-C11
|
|
交叉反应
|
Mouse ; Human
|
|
蛋白分子量
|
60kDa
|
|
应用
|
IF ; ICC ; WB
|
|
稀释比例
|
WB 1:500-1000. IF 1:50-200. ICC 1:50-200.
|
|
免疫原
|
Recombinant protein of human Smad2
|
|
纯化方法
|
Affinity purification
|
|
储存液
|
Buffer: PBS with 0.02% sodium azide, 0.5% BSA, 50% glycerol, pH7.3.
|
|
保存
|
Store at -20℃. Avoid freeze / thaw cycles.
|
|
规格
|
100ul ; 50ul
|
|
单位
|
支
|
These protocols are for reference only. Solarbio does not independently validate these methods.
Quick spin before use. If you have any questions, please contact us.
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
a simple ELISA method to detect human anti-antibody response.
