DNA METHYLTRANSFERASE 1 ANTIBO

DNA-Methyltransferase Assay

of endogenous DNA.By the same token,a higher cellular protein concentration will also lead to a higher negative control for similar reasons ( protein methylation). If your positive controls are too low: Did you deviate from protocol? (e.g.,not drying

DNA-Methyltransferase Assay

in Econofluor) Also, a longer NaOH incubation might reduce this problem some. But remember, the higher the activity, the higher the negative control will be because of methylation of endogenous DNA. By the same token, a higher cellular protein concentration

DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo

for studying DNA?protein interaction in vivo. A mutant form of DNA adenine methyltransferase (DamK9A) from E. coli is fused to the protein of interest and expressed. The fusion protein will bind to target binding sites and introduce N 6 ?adenine methylation