HEAT SHOCK PROTEIN 90 BETA ANT

Transcription, Translation of S35-Radiolabelled Protein and Binding to GST-Fused Proteins or Antibodies (IP)

reticulocyte lysate. S35 methionine is DuPont translation grade (5mCi/mmol) Heat RNA 60°C 5 min, ice and prepare reaction cocktail. The lysate can be refrozen once. Only prepare a cocktail with the amount required. For example

secondary antibody review -- data from 99 publications

cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

稳定表达具有生物活性的人重组对氧磷酶

by western blot analysis using an anti-paraoxonase-1 antibody.   Large-scale expression and harvesting of paraoxonase-1 protein Positive foci were identified and seeded into expansion cultures containing media supplemented with selection antibiotic