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- 钦诚生物
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- 2025年07月15日
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文献和实验Real-Time PCR: the TaqMan Method
, each with their advantages. This web site will explore one of these types, TaqMan® real-time PCR, as well as give an overview of the other two types of real-time PCR, molecular beacon and SYBR® Green. ( www.ambion.com 2003) How TaqMan® works: TaqMan®
TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
in the amount of PCR product is quantified in real time as the special probes, "reporter dye", fluoresces when the "quencher" is removed from the fragment during the PCR extension cycle.Application of the "TaqMan® Probe" to Gene expression problemsTo induce
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