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E.coli Enoyl-ACP Reductase / F

ABI Protein (His Tag)
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  • ¥2159.15
  • Frdbio
  • WUHAN,CHINA
  • FrdE1225-801E
  • 2025年11月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      <- 20℃

    • 保质期

      >2 years

    • 英文名

      Friendbio Science & Technology Co.,Ltd.

    • 库存

      200

    • 供应商

      福因德科技(武汉)有限公司

    • 规格

      50μg

    分子别名(Synonym):b1288,ECK1283,envM,gts,JW1281,qmeA 种属(Species):E. coli 表达系统(Expressed Host):E. coli 制剂(Buffer):Lyophilized from sterile 20mM Tris 0.5M NaCl, 10% glycerol, pH 7.5 分子量(Molecule Mass):The recombinant E.coli FABI consisting of 276 amino acids and has a calculated molecular mass of 29.5 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions. 内毒素(Endotoxin):<1.0 EU per μg by the LAL method. 生物活性(Activity): 表达区间(Protein Construction):A DNA sequence encoding the E.coli FABI (NP_287908.1) (Gly 2-Lys 262) was expressed, with a polyhistide tag at the N-terminus. 存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution. 运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

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    图标文献和实验
    相关实验
    • Tag不留痕――NEB原核表达系列

      Binding Protein,MBP)的大肠杆菌malE基因,其下游的多克隆位点便于目的基因插入,表达N端带有MBP的融合蛋白。此系统的pMAL载体除了含有氨苄霉素抗性,还含有LacZα基因,目的基因的插入导致其失活,通过X-gal平板可进行蓝白斑筛选。pMAL载体都含有一段编码蛋白酶识别位点的序列,融合蛋白纯化后,通过Factor Xa(X),Enterokinase (E)或 Genenase™ I (G)可将目的蛋白与MBP切割分离。如果不想在切割后留下任何外源氨基酸可以在设计引物时引入XmnⅠ

    • 凝胶延长分析二型脂肪酸合成的方法

      to produce acetoacetyl-ACP. This product is reduced by FabG, dehydrated by FabA/Z to produce C4:1(Δ2)-ACP, and then reduced second time by FabI to make butyryl-ACP (C4:0-ACP)which is substrate for FabF/B condensing enzymes. Cerulenin, a selective FabF/B

    • Gel-elongation assay for type II fatty acid synthesis

      In bacteria, FabH catalyzes the first condensation reaction using acetyl-CoA and malonyl-ACP to produce acetoacetyl-ACP. This product is reduced by FabG, dehydrated by FabA/Z to produce C4:1(Δ2)-ACP, and then reduced second time by FabI to make

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