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- 文献和实验
- 技术资料
- 保存条件:
<- 20℃
- 保质期:
>2 years
- 英文名:
Friendbio Science & Technology Co.,Ltd.
- 库存:
200
- 供应商:
福因德科技(武汉)有限公司
- 规格:
50μg
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文献和实验AFLP: not only for fingerprinting, but for positional cloning
) 1.2 µL 25 mM MgCl2 0.08 µL (0.4 U) Taq Pol (Promega) 13.82 µL dH20 ‡ The basic MseI-primer is: 5'-GAT GAG TCC TGA GTA Axy z-3' like with the EcoRI-primer, x, y, and z represent selective bases that are specific and constant
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
, these proteins must be supplied through complementation. The products of the genes, gag, pro, pol, and env are typically supplied by "packaging" cell lines or cotransfection with packaging constructs into highly transfectable cell lines (for review see Cepko
. Liu X and Erikson RL. (2002). Activation of Cdc2/cyclin B and inhibition of centromere amplification in cells depleted of Plk1 by siRNA. Proc. Natl. Acad. Sci. USA 99(13):8672-8676. Jiang M and Milner J. (2002). Selective silencing of viral gene
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