Mouse IL6ST / gp130 / CD130 Pr

分子别名（Synonym）:5133400A03Rik,AA389424,BB405851,CD130,D13Ertd699e,gp130 种属（Species）：Mouse 表达系统（Expressed Host）：HEK293 Cells 制剂（Buffer）：Lyophilized from sterile PBS, pH 7.4 分子量（Molecule Mass）：The recombinant mouse gp130/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 843 amino acids and has a predicted molecular mass of 94.7 kDa. As a result of glycosylation, the apparent molecular mass of rm gp130/Fc monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions. 内毒素（Endotoxin）：＜1.0 EU per μg by the LAL method. 生物活性(Activity)：Measured by its ability to inhibit the IL6 Rα enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168.). The ED50 for this effect is typically 0.03-0.15 μg/ml in the presence of 10 ng/ml recombinant human IL6sR and 20 ng/ml recombinant human IL6. 表达区间（Protein Construction）：A DNA sequence encoding the mouse gp130 (NP_034690.3) extracellular domain (Ser 2-Glu 617) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. 存储条件（Storage）： For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution. 运输条件： In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.