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Human TGFBR1 / ALK-5 / SKR4 Pr

otein (His & Fc Tag)
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  • ¥3014.85
  • Frdbio
  • WUHAN,CHINA
  • FrdH1683-203H
  • 2025年11月04日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      <- 20℃

    • 保质期

      >2 years

    • 英文名

      Friendbio Science & Technology Co.,Ltd.

    • 库存

      200

    • 供应商

      福因德科技(武汉)有限公司

    • 规格

      100μg

    分子别名(Synonym):AAT5,ACVRLK4,ALK-5,ALK5,ESS1,LDS1,LDS1A,LDS2A,MSSE,SKR4,tbetaR-I,TGFR-1 种属(Species):Human 表达系统(Expressed Host):HEK293 Cells 制剂(Buffer):Lyophilized from sterile PBS, pH 7.4 分子量(Molecule Mass):The recombinant human TGFBR1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 349 amino acids and has a predicted molecular mass of 38.8 kDa. As a result of glycosylation, the apparent molecular mass of rh TGFBR1/Fc monomer is approximately 45-50 kDa in SDS-PAGE under reducing conditions, with ~10% free Fc fragments. 内毒素(Endotoxin):<1.0 EU per μg by the LAL method. 生物活性(Activity):1. Measured by its binding ability in a functional ELISA 表达区间(Protein Construction):A DNA sequence encoding the human TGFBR1 (NP_004603.1) extracellular domain (Ser 2-Glu 125) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. 存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution. 运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

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    • Adeno-X表达系统

      载体克隆到腺病毒载体 -- 0-15天 4天 获得重组腺病毒 14-21天 17-22天 4-7天 噬斑纯化 5-10天 0-5天 -- 总计 26-45天 24-56天 10-17天     以体外连接方法为基础 (by Mizuguchi和Kay),CLONTECH的Adeno-X™表达系统提供了一种快速、高效构建重组腺病毒的方法。只需4至7天就可以获得重组腺病毒,如图1所示。由于经过7天的培养之后HEK 293细胞会渐渐失去对培养皿的附着而扩散,导致噬斑融合,用传统方法制备重组腺病毒往往需要铺平

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    ¥3014.85