人参皂苷Rd 分析对照品

使用本产品的案例（仅供参考）

UPLC

The experimental conditions were as follows: 1) Column: Waters Acquity BEH C18 column (100 mmX2.1 mm, 1.7 mm); 2) chromatographic conditions were: flow rate 0.3 mL/ min; detection wavelength (k) ¼ 203 nm; sample volume 5 mL; column temperature 30 ℃. With acetonitrile (B) and 0.1% phosphoric acid solution (D) as mobile phase, gradient elution: 0–3 min, 19% B; 3–5 min, 20% B; 5–12 min, 21% B; 12–13 min, 29% B; 13–25 min, 31% B; 25–32 min, 35% B; 32–35 min, 19% B; 35–41 min, 19% B

来源文献：Wu SH, Li HB, Li GL, Qi YJ, Zhang J, Wang BY. Panax ginseng root, not leaf, can enhance thermogenic capacity and mitochondrial function in mice. Pharm Biol. 2020 Dec;58(1):374-384. doi: 10.1080/13880209.2020.1756348. PMID: 32366153; PMCID: PMC7241452.

UPLC

Concentration analysis of ginsenoside was conducted on ACQUITY UPLC system (Waters, Milford, MA) equipped with diode array detector (190 to 800 nm) and a reversed-phase C18 column (2.1 × 50 mm; inner diameter 1.7 μm; Acquity UPLC BEH; Waters). The mobile phase consisted of ACN (A) and 0.00005‰ (v/v) phosphoric acid (B) with the following separation program based on Zhang et al (2018): 0 to 3 min, 17 to 19% A; 3 to 4 min, 19 to 21% A; 4 to 4.5 min, 21 to 24% A; 4.5 to 5 min, 24 to 28% A; 5 to 6.5 min, 28% A; 6.5 to 7.5 min, 28 to 30% A; 7.5 to 9.5 min, 30 to 36% A; 9.5 to 11 min, 36 to 40% A.The injection volume was 2 μL at a flow rate of 0.5 mL min−1 . The column temperature and the detection wavelength were respectively set up at 35°C and 203 nm.

来源文献：[1] Lei X , Wang Q , Yang H ,et al.Vitrification and proteomic analysis of embryogenic callus ofPanax ginsengC. A. Meyer[J].In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association, 2021(1):57.