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- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 保质期：
6个月
- 英文名：
Cellulase(CL) Activity Assay Kit
- 库存：
99
- 供应商：
北京索莱宝科技有限公司
- CAS号：
BC2545-100T/48S
- 规格：
100管/48样
产品组成：使用前请认真核对试剂体积与瓶内体积是否一致，有疑问请及时联系索莱宝工作人员。
| 试剂名称 | 规格 | 保存条件 |
| 提取液 | 液体60 mL×1瓶 | 2-8℃保存 |
| 试剂一 | 液体5 mL×1瓶 | 2-8℃保存 |
| 试剂二 | 液体20 mL×1瓶 | 2-8℃保存 |
| 试剂三 | 液体5 mL×1瓶 | 2-8℃保存 |
| 标准品 | 粉剂×1支 | 2-8℃保存 |
溶液的配制：
标准品：10mg无水葡萄糖（干燥失重<0.2%）。临用前加入1mL蒸馏水溶解，配制成10mg/mL葡萄糖溶液备用，2-8℃保存两周，或者用饱和苯甲酸溶液溶解，可保存更长时间。
产品说明：
CL（EC 3.2.1.4）存在于细菌、真菌和动物体内，能够催化纤维素降解，是一类可广泛应用于医药、食品、棉纺、环保及可再生资源利用等领域的酶制剂。
采用3,5-二硝基水杨酸法测定CL催化纤维素降解产生的还原糖的含量。
注意：实验之前建议选择2-3个预期差异大的样本做预实验。如果样本吸光值不在测量范围内建议稀释或者增加样本量进行检测。
需自备的仪器和用品：
可见分光光度计/酶标仪、水浴锅、低温离心机、可调式移液器、微量玻璃比色皿/96孔板、研钵/匀浆器、冰和蒸馏水。
操作步骤：具体操作步骤详见“产品资料”附件
一、样本处理（可适当调整待测样本量，具体比例可以参考文献）
- 细菌或细胞：收集细菌或细胞到离心管内，离心后弃上清；按照每500万细菌或细胞加入1mL提取液，超声冰浴破碎细菌或细胞（功率200w，超声3秒，间隔10秒，重复30次）；8000g 4℃离心10min，取上清，置冰上待测。
- 组织：称取约0.1g组织加入1mL提取液，冰浴中匀浆。8000g ，4℃离心10min，取上清，置冰上待测。
二、测定步骤
相关发表文献：
Guo Q, Du G, Qi H, et al. A nematicidal tannin from Punica granatum L. rind and its physiological effect on pine wood nematode (Bursaphelenchus xylophilus)[J]. Pesticide biochemistry and physiology, 2017, 135: 64-68.
参考文献：
Faria M L, Kolling D, Camassola M, et al. Comparison of Pennicillium echinulatum and Trichoderma reesei cellulases in relation to their activity against various cellulosic substrates[J]. Biores. Technol, 2008, 99: 1417-1424. 
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（一）检测原理 血细胞比容（Hct、Ht、HCT或PCV）是指在一定条件下，经离心沉淀压紧的红细胞在全血样本中所占比值。 1、离心法：包括温氏法（Wintrobe法）、微量法：是将抗凝血置于孔径统一的温氏管或毛细玻管中，以一定转速离心一定时间后，计算红细胞层占全血的体积比。 2、血液分析仪法：原理是当细胞通过计数小孔时，形成相应大小的脉冲，脉冲的多少即为细胞数量，脉冲高度为细胞体积，通过红细胞平均体积（MCV）和红细胞计数（RBC）即求得血细胞比容，Hct=MCV×RBC
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1、手工法：有折射计法、粘度法、比密测定法、离心法和放射性核素法。温氏法：采用中速离心，不能完全排除红细胞间残留血浆，测定结果偏高，已属淘汰。微量法：采用高速离心，细胞间残留血浆比温氏法少（约2%），且样本用量小、操作简便、残留血浆1%～3%。 2、血液分析仪法：仪器法CV为1%，手工法CV为2%，仪器法应注意红细胞增多症或血浆渗透压异常时会出现误差。
