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- 用户评价
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 保质期：
12个月
- 英文名：
Soil Catalase(S-CAT) Activity Assay Kit
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- CAS号：
BC0105-100T/48S
- 规格：
100管/48样
土壤过氧化氢酶（S-CAT）活性检测试剂盒说明书
微量法
货号：BC0105
规格：100T/48S
产品组成：使用前请认真核对试剂体积与瓶内体积是否一致，有疑问请及时联系索莱宝工作人员。
| 试剂名称 | 规格 | 保存条件 |
| 试剂一 | 液体0.3 mL×1瓶 | 2-8℃保存 |
| 试剂二 | 粉剂×1支 | 2-8℃保存 |
| 试剂三 | 液体3 mL×1瓶 | 2-8℃保存 |
溶液的配制：
- 试剂一：液体置于试剂瓶内EP管中，使用前需将EP管先离心。临用前取0.05 mL试剂一加入9.95 mL蒸馏水稀释待用或者按比例配制。用不完的试剂2-8℃保存1周；
- 试剂二：临用前加入1 mL 蒸馏水充分溶解待用，用不完的试剂2-8℃保存4周。
产品说明：
S-CAT是土壤微生物代谢的重要酶类，在H2O2清除系统中具有重要作用。
H2O2在240nm下有特征吸收峰，通过测定与土壤反应后溶液在此波长下吸光度的变化，即可反应S-CAT活性的高低。
注意：实验之前建议选择2-3个预期差异大的样本做预实验。如果样本吸光值不在测量范围内建议稀释或者增加样本量进行检测。
需自备的仪器和用品：
紫外分光光度计/酶标仪、台式离心机、水浴锅、可调式移液器、微量石英比色皿/96 孔UV板、研钵、30~50目筛和蒸馏水。
操作步骤：具体操作步骤详见“产品资料”附件
注意事项：
如果吸取的上清仍有部分浑浊，可以在加入试剂三后统一再次进行离心。
相关发表文献：
Hou Q, Wang W, Yang Y, et al. Rhizosphere microbial diversity and community dynamics during potato cultivation[J]. European Journal of Soil Biology, 2020, 98: 103176.
参考文献：
[1] 杨兰芳, 曾巧, 李海波, et al. 紫外分光光度法测定土壤过氧化氢酶活性[J]. 土壤通报, 2011, 42(1):207-210.
[2] Johansson L H, Borg L A H. A spectrophotometric method for determination of catalase activity in small tissue samples[J]. Analytical biochemistry, 1988, 174(1): 331-336.
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