- $665
- LSBio
- 78.1 - 5000 pg/ml
- LS-F23974
- 美国
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
78.1 - 5000 pg/ml
- 供应商：
康朗生物
- 规格：
96T
Human NOS2 / iNOS ELISA Kit (Sandwich ELISA) - LS-F23974
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Product Description
LS-F23974 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human NOS2 / iNOS in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of NOS2 / iNOS as low as 78.1 picograms per milliliter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
NOS2 / iNOS
Reactivity
Human
Specificity
This kit recognizes natural and recombinant Human NOS2/iNOS.
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
78.1 - 5000 pg/ml
Sensitivity
78.1 pg/ml
Assay Time
3.5 h
Precision
Intra-Assay: CV<5.6% Inter-Assay: CV<6.51%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Protocol
- Prepare all reagents, samples and standards.
- Add 100 µl standard or sample to each well and incubate for 90 minutes at 37°C.
- Aspirate and add 100 µl prepared Detection Antibody and incubate of 1 hour at 37°C.
- Aspirate and wash 3 times.
- Add 100 µl HRP Conjugate and incubate for 30 minutes at 37°C.
- Aspirate and wash 5 times.
- Add 90 µl Substrate Solution and incubate for 15-20 minutes at 37°C.
- Add 50 µl Stop Solution.
- Read immediately at 450nm.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About NOS2 / iNOS
P35228 NM_000625 NP_000616.3
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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