Mouse Histocompatibility 2, Cl
ass II antigen A, alpha (H2-Aa) ELISA Kit (Competitive EIA) - LS-F40022- $715
- LSBio
- 0.625 - 40 ng/ml
- LS-F40022
- 美国
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
0.625 - 40 ng/ml
- 供应商：
康朗生物
- 规格：
96T
Mouse Histocompatibility 2, Class II antigen A, alpha (H2-Aa) ELISA Kit (Competitive EIA) - LS-F40022
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Product Description
LS-F40022 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse Mouse Histocompatibility 2, Class II antigen A, alpha (H2-Aa) in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of Mouse Histocompatibility 2, Class II antigen A, alpha (H2-Aa) as low as 0.375 nanograms per millilter.Specifications
TypeCompetitive EIA (enzyme immunoassay) kit
Target
Mouse Histocompatibility 2, Class II antigen A, alpha (H2-Aa)
Reactivity
Mouse
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.625 - 40 ng/ml
Sensitivity
0.375 ng/ml
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Store at 4°C. Stable for 6 months.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Background
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with H2-Aa. During the reaction, H2-Aa in the sample or standard competes with a fixed amount of H2-Aa on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to H2-Aa. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of H2-Aa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About Mouse Histocompatibility 2, Class II antigen A, alpha (H2-Aa)
NM_010378
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Competition ELISA Platform Overview
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(1 –3 ). Most T cells are specific for peptide epitopes in association with either classical MHC class Ia molecules (HLA-A, B, and C in humans and H2-K, D, and L in mice) in the case of CD8+ T cells, or class II molecules (HLA-DR, DP, and DQ in humans
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