- $865
- LSBio
- 1.563 - 100 ng/ml
- LS-F36802
- 美国
- 2025年07月11日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Monkey
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
1.563 - 100 ng/ml
- 供应商：
康朗生物
- 规格：
96T
Monkey Membrane IgM ELISA Kit (Sandwich ELISA) - LS-F36802
Print This Page
Technical Help
Review This Product
SpecificationsPublicationsReviewsImages
Product Description
LS-F36802 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Monkey Membrane IgM in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Membrane IgM as low as 0.938 nanograms per millilter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
Membrane IgM
Reactivity
Monkey
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
1.563 - 100 ng/ml
Sensitivity
0.938 ng/ml
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Store at 4°C. Stable for 6 months.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- mIgM antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-mIgM antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mIgM amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofmIgM can be calculated.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
Popular Membrane IgM Elisa Kits
Human Membrane IgM ELISA Kit (CLIA) - LS-F29728
Reactivity: Human
Range: 0.938-60 ng/ml
Human Membrane IgM ELISA Kit (Sandwich ELISA) - LS-F37472
Reactivity: Human
Range: 3.125-200 ng/ml
Monkey Membrane IgM ELISA Kit (Custom ELISA) - LS-F45642
Reactivity: Monkey
Range: 1.563-100 ng/ml
Chicken Membrane IgM ELISA Kit (Custom ELISA) - LS-F45643
Reactivity: Chicken
Range: 3.125-200 ng/ml
More Membrane IgM Products
Publications (0)
Submit a Publication
Customer Reviews (0)
Submit a Review
Top of Page
Images
Sandwich ELISA Platform Overview
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
]=2.5 单抗HRPO标记是否成功？ 直观验证： 七、HSV1单克隆抗体的应用 血清中IgM抗体的测定： 血清中IgM抗体的检测用于传染病的早期诊断中。 间接法ELISA一般仅适用于检测总抗体或IgG抗体。 血清中针对某抗原的IgM和IgG同时存在，大量存在的IgG会干扰IgM抗体的测定。 间接ELISA测定IgM抗体，必须先将标本用A蛋白处理或抗IgG抗体中和，以除去IgG的干扰。 捕获法ELISA： 在临床检验中测定
摘要 目的 探讨循环免疫复合物与脑梗死的关系。方法 采用酶联免疫吸附试验（ELISA）方法测定脑梗死患者血中巨细胞病毒抗体（CMV-IgG与CMV-IgM）及衣原体抗体（CP-IgG），并与健康对照组比较。结果 脑梗死组循环免疫复合物显著升高（P﹤0.01），其中CMV-IgM在发病早期明显升高(P﹤0.01)，随着病情康复，其水平逐渐下降。CMV-IgG和CP-IgG也呈现类似变化。结论 脑梗死患者血中CMV-IgG、CMV-IgM和CD-IgG升高，循环复合物对脑梗死的发生有一定的不良
技术资料暂无技术资料 索取技术资料
