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文献和实验SequalPrep Long PCR Kit with dNTPs
实验步骤 1. Program the thermal cycler as follows (note that the annealing temperature will vary depending on the Tm of your primers): 94°C 2 minutes 10 cycles
. Lysis buffer (store on the benchtop without proteinase K). 60 g/ml proteinase K 10mM Tris-Cl, pH 8.2 50mM KCl 2.5mM MgCl2 0.45% Tween-20 0.05% gelatin Master mix . Prepare a master mix containing the following
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Technologies; Cat # 48190-011) Cyanine 3-dUTP # NEL578 or Cyanine 5-dUTP #NEL579 (Perkin Elmer/NEN) B. REAGENT PREPARATION 50X dNTPs 50X [ ] Stock [ ] For 1.0 ml of 50X use:
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