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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
for future use below -18°C
- 保质期:
See instructions
- 英文名:
GroES
- 库存:
常规产品有备货
- 供应商:
上海经科化学科技有限公司
- CAS号:
无
- 规格:
10ug/50ug/1mg
| 规格: | 10ug | 产品价格: | ¥1080.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥2415.0 |
| 规格: | 1mg | 产品价格: | ¥30429.0 |

CATALOGUE NUMBER
HSP-005
SYNONYMS
INTRODUCTION
DESCRIPTION
SOURCE
PHYSICAL APPEARANCE
FORMULATION
STABILITY
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
PURITY
AMINO ACID SEQUENCE
SAFETY DATA SHEET
SDS
USAGE
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文献和实验Recombinant GroES produced in E.Coli is a single, non-glycosylated polypeptide chain containing 97 amino acids and having a molecular mass of 10.4 kDa.
Generation of Recombinant Baculovirus DNA in E.coli Using a Baculovirus Shuttle Vector
of the Autographa californica nuclear polyhedrosis virus (AcNPV) are abundantly expressed during the late stages of infection. The recombinant proteins are usually soluble and functionally similar to their authentic counterparts (l –7 ). In the following sections
Maxiprep of plasmid DNA from E.coli protocol
Solutions/reagents: LB broth + selective marker 50% sterile glycerol TEG (25mM Tris-Cl, 10mM EDTA, 50mM dextrose) 20 mg/ml lysozyme 10% SDS 4M NaOH autoclaved water Solution 3 (3M potassium-acetate, 2M acetic acid
E.coli genomic library construct 求教
) 5. ethanol 沉淀,electrotransformation, 结果得到的colony比较少,0.5ug 连接产物沉淀后, dissolve in 20ul ddH2O, 2ul + 50ul cell, spread on 8 plates, 总共只得到约1000个colonies(由于vector是单酶切,这里面还有载体自连产物,电转感受态细胞的转化效率很高,10的7次方-10的8次方),我的目的是希望尽可能cover E.coli 2kb-5kb之间的gene,所以希望能够得到5000-1w左右
技术资料







