Recombinant Human Ribosomal Ph

Ribosomal protein large P2, RPP2, MGC71408, P2, Renal carcinoma antigen NY-REN-44, D11S2243E, 60S acidic ribosomal protein P2, Acidic Ribosomal phosphoprotein P2.

Ribosomes, the organelles which catalyze protein synthesis, contain a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and nearly 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein which is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, is a member of the L12P family of ribosomal proteins and has a vital part in the elongation step of protein synthesis. In opposed to most ribosomal proteins, which are basic, the encoded protein is acidic.The P1 C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P2. The P1 protein can interact with P0 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. P1 is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene distributed all over the genome.

RPLP2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 139 amino acids (1-115 a.a.) and having a molecular mass of 14.2kDa.
RPLP2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile filtered colorless solution.

RPLP2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 1mM DTT and 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Greater than 95% as determined by SDS-PAGE.

MG.S.SHHHHHH SSGLVPRGSH MGSHMRYVAS YLLAALGGNS SPSAKDIKKI LDSVGIEADD DRLNKVISEL NGKNIEDVIA QGIGKLASVP AGGAVAVSAA PGSAAPAAGS APAAAEEKKD EKKEESEESD DDMGFGLFD

SDS

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