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Recombinant Protein G His Tag

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  • ¥1080 - 25000
  • Prospecbio
  • 以色列
  • pro-1237
  • 2026年01月22日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      Protein-G His

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      1mg/10mg/100mg

    规格:1mg产品价格:¥1080.0
    规格:10mg产品价格:¥4500.0
    规格:100mg产品价格:¥25000.0

    产品细节图片1

    CATALOGUE NUMBER

    PRO-1237

    DESCRIPTION

    Protein G His Tag Recombinant produced in E.Coli is a 201 amino acids protein which contains amino acid 190-384 of the Streptococcus sp with a C-terminal 6-His tag, and having a molecular mass of 21.6kDa. But it migrates with an apparent molecular mass of 32kDa in SDS-PAGE.
    The Protein G His Tag is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    Lyophilized with no additives.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized Protein G in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized Protein G although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Protein G should be stored at 4°C between 2-7 days and for future use below -18°C.
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 95.0% as determined by SDS-PAGE and HPLC analyses.

    SPECIFICITY

    1. Binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a.
    2. Does not bind to human IgM, IgD and IgA.

    APPLICATIONS

    Protein G binds to the constant region of many species of immunoglobulin G. It can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
     

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    图标文献和实验
    该产品被引用文献

    Protein G His Tag Recombinant produced in E.Coli is a 201 amino acids protein which contains amino acid 190-384 of the Streptococcus sp with a C-terminal 6-His tag, and having a molecular mass of 21.6kDa. But it migrates with an apparent molecular mass of 32kDa in SDS-PAGE.
    The Protein G His Tag is purified by proprietary chromatographic techniques.

    相关实验
    • ANTIBODY BINDING TO PROTEIN A AND PROTEIN G

      of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G

    • Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors

      The molecular cloning of the cDNA sequences encoding most G-protein-coupled receptors, including those from humans, allows their study in a variety of recombinant systems. In this respect, transfected mammalian cell lines constitute

    • Recombinant DNA Engineering, Or Cloning Genes In Plasmids

      and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal

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    文献支持
    Recombinant Protein G His Tag
    ¥1080 - 25000