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- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
MYL1
- 库存：
常规产品有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
2ug/10ug/1mg
| 规格： | 2ug | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 10ug | 产品价格： | ￥2415.0 |
| 规格： | 1mg | 产品价格： | ￥52000.0 |
CATALOGUE NUMBER
PRO-365
SYNONYMS
INTRODUCTION
MYL1 gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for the MYL1 gene. In humans MYL1 is localized to chromosome 2q32.1-qter. The Myl1 locus encodes two alkali myosin light chains- Mlc1f and Mlc3f, from two promoters that are differentially regulated throughout development. The Mlc1f promoter is active in embryonic, fetal and adult fast skeletal muscle while the Mlc3f promoter is upregulated during fetal development and stays on in adult fast skeletal muscle.
DESCRIPTION
SOURCE
PHYSICAL APPEARANCE
FORMULATION
STABILITY
Please prevent freeze-thaw cycles.
PURITY
SAFETY DATA SHEET
SDS
USAGE
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文献和实验Recombinant Human Ventricular Myosin Light Chain-1 (MYL1) protein has a molecular mass of 25 kDa and is fused to 7 amino acids at N-terminus. The MYL1 protein was affinity purified using anti MYL1 monoclonal antibody 39-15 column.
Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
构成肌球蛋白的低分子量（1.5— 2.5万）的亚单位。一分子肌球蛋白有 4个。肌球蛋白有在腺苷三磷酸酶（ ATP ase）作用中不可缺少的基本 L链和调节 ATP ase作用的调节 L链。从兔的白肌除去调节 L链（ L2 ：由 168个氨基酸所成）不影响肌球蛋白的作用。基本 L链（ L1 ∶ 190个氨基酸、 L3 ∶ 149个氨基酸）是必要的。兔白肌的肌球蛋白分子，根据 L链的不同而分为 3种，即每分子肌球蛋白中都含有两个 L2 ，其它两个则因种类不同而异，即分 L1
技术资料