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Recombinant Human Cytokeratin

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  • ￥1080 - 87906
  • Prospecbio
  • 以色列
  • pro-347
  • 2026年01月17日
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    上海经科化学科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      KRT8

    • 库存：

      常规产品有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      5ug/20ug/1mg

    规格：5ug产品价格：￥1080.0
    规格：20ug产品价格：￥2415.0
    规格：1mg产品价格：￥87906.0

    产品细节图片1

    CATALOGUE NUMBER

    PRO-347

    SYNONYMS

    Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CYK8, KO, CK8, K2C8, CARD2.

    INTRODUCTION

    Keratin 8 and 18 (K8/18) are the major components of intermediate filament (IF) proteins of simple or single-layered epithelia.

    DESCRIPTION

    Cytokeratin 8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 53,532 Dalton.
    The KRT8 is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCl pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized KRT8 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized KRT8 although stable at room temperature for 3 weeks, should be stored at 2-8°C. Upon reconstitution KRT8 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    PURITY

    Greater than 95.0% as determined by:
    (a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.

    RECONSTITUTION TO FILAMENTS

    Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

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    图标文献和实验
    该产品被引用文献

    1.Title:Role for the PP2A/B56? Phosphatase in Regulating 14-3-3 Release from Cdc25 to Control Mitosis.
    Publication:Received 3 May 2006. Revised 23 August 2006. Accepted 9 October 2006. Available online 16 November 2006. Published: November 16, 2006.

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    文献支持
    Recombinant Human Cytokeratin 8
    ￥1080 - 87906