产品封面图
文献支持

Recombinant Human Cytokeratin

18
收藏
  • ¥1080 - 87906
  • Prospecbio
  • 以色列
  • pro-349
  • 2026年01月17日
    avatar
  • 企业认证

    点击 QQ 联系

    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      KRT18

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      5ug/20ug/1mg

    规格:5ug产品价格:¥1080.0
    规格:20ug产品价格:¥2415.0
    规格:1mg产品价格:¥87906.0

    产品细节图片1

    CATALOGUE NUMBER

    PRO-349

    SYNONYMS

    Keratin type I cytoskeletal 18, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18,CYK18,Cell proliferation-inducing gene 46 protein.

    INTRODUCTION

    KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

    DESCRIPTION

    Cytokeratin 18 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain having a calculated molecular mass of 48,201 Dalton, showing a 45kDa band on SDS-page, pI-5.7.
    The KRT18 is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    The protein (1mg/ml) was lyophilized from a sterile solution containing 30mM Tris-HCl pH-8, 9.5M urea, 2mM EDTA, 2mM DTT and 10mM methylammonium chloride.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized 1mg CK-18 in sterile 18MΩ-cm H2O not less than 700µl, which can then be further diluted to other aqueous solutions. 

    STABILITY

    Lyophilized CK-18 although stable at room temperature for 3 weeks, should be stored between 2-8°C. Upon reconstitution CK-18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    PURITY

    Greater than 95.0% as determined by analysis by SDS-PAGE.

    RECONSTITUTION TO FILAMENTS

    Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    SAFETY DATA SHEET

    SDS

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    该产品被引用文献

    1.Title:Role for the PP2A/B56 Phosphatase in Regulating 14-3-3 Release from Cdc25 to Control Mitosis.
    Publication:Copyright 2006 Elsevier Inc. All rights reserved. Cell, 759-773, 17 November 2006 doi:10.1016/j.cell.2006.10.035

    2.Title:Role for the PP2A/B56? Phosphatase in Regulating 14-3-3 Release from Cdc25 to Control Mitosis.
    Publication:
    Received 3 May 2006. Revised 23 August 2006. Accepted 9 October 2006. Available online 16 November 2006. Published: November 16, 2006.

    相关实验
    • Construction of Recombinant Human Cytomegalovirus

      The use of reverse genetics to generate recombinant viruses allows the researcher to investigate the exact functional significance of particular viral genes during the virus life cycle, by means of their deletion or modification in the viral

    • Delivery of Recombinant Adenoviruses to Human Saphenous Vein

      The human saphenous vein is the most commonly used conduit for coronary artery bypass grafting owing to its ready availability, ease of harvesting, and favorable surgical handling (1 ). However, it suffers from a progressive decline

    • Recombinant Genetic Libraries and Human Monoclonal Antibodies

      In order to comprehensively manipulate the human proteome we require a vast repertoire of pharmacological reagents. To address these needs we have developed repertoires of synthetic antibodies by phage display, where diversified

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    ¥987
    爱必信(上海)生物科技有限公司
    2025年07月14日询价
    ¥1080
    上海经科化学科技有限公司
    2026年01月17日询价
    询价
    默瑞(上海)生物科技有限公司
    2025年07月15日询价
    ¥3017
    广州威佳科技有限公司
    2025年07月09日询价
    ¥30662
    上海北诺生物科技有限公司
    2025年07月16日询价
    文献支持
    Recombinant Human Cytokeratin 18
    ¥1080 - 87906