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Recombinant Human Charged Mult

ivesicular Body Protein 6
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  • ¥1080 - 45885
  • Prospecbio
  • 以色列
  • pro-1085
  • 2026年01月14日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      CHMP6

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      5ug/20ug/1mg

    规格:5ug产品价格:¥1080.0
    规格:20ug产品价格:¥2415.0
    规格:1mg产品价格:¥45885.0

    产品细节图片1

    CATALOGUE NUMBER

    PRO-1085

    SYNONYMS

    Charged multivesicular body protein 6, Chromatin-modifying protein 6, Vacuolar protein sorting-associated protein 20, Vps20, hVps20, CHMP6.

    INTRODUCTION

    Charged multivesicular body protein 6 (CHMP6) is a member of the SNF7 family. The CHMP6 protein is a core component of the endosomal sorting necessary for transport complex III (ESCRT-III) that is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) which are produced by invagination and scission from the limiting membrane of the endosome and generally are transported to lysosomes facilitating degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids.

    DESCRIPTION

    CHMP6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-201 a.a) and having a molecular mass of 26.1kDa (Molecular size on SDS-PAGE will appear higher).
    CHMP6 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered colorless solution.

    FORMULATION

    CHMP6 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol and 100mM NaCl.

    STABILITY

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Avoid multiple freeze-thaw cycles.

    PURITY

    Greater than 95.0% as determined by SDS-PAGE.

    AMINO ACID SEQUENCE

    MG.S/SHHHHHH SSGLVPRGSH MGSHMGNLFG RKKQSRVTEQ DKAILQLKQQ RDKLRQYQKR IAQQLERERA LARQLLRDGR KERAKLLLKK KRYQEQLLDR TENQISSLEA MVQSIEFTQI EMKVMEGLQF GNECLNKMHQ VMSIEEVERI LDETQEAVEY QRQIDELLAG SFTQEDEDAI LEELSAITQE QIELPEVPSE PLPEKIPENV PVKARPRQAE LVAAS.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
     
     

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    图标文献和实验
    该产品被引用文献

    CHMP6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-201 a.a) and having a molecular mass of 26.1kDa (Molecular size on SDS-PAGE will appear higher).
    CHMP6 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    相关实验
    • Protein Folding Liquid Chromatography

      and a new chromatographic cake. A protocol for fast renaturation with simultaneous purification of inclusion body protein of the recombinant human interferon-gamma to obtain purity ≥95% and high specific bioactivity in a single step and in 1 h is introduced.

    • Introduction of human 14-3-3 proteins

      isolated from eight different human tissues shows that 14-3-3 protein mRNAs are expressed in many tissues in the body.In agreement with previous reports,the highest abundance of RNA hybridizing with this cDNA is seen in the brain.----

    • Strategies to Optimize Protein Expression in E. coli

      . 2009a. Recombinant expression and affinity purification of a novel epididymal human sperm‐binding protein, BSPH1. Mol. Hum. Reprod. 15:105‐114.    Lefebvre, J., Boileau, G

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    文献支持
    Recombinant Human Charged Multivesicular Body Protein 6
    ¥1080 - 45885