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Recombinant M.ycoba.cteriu.m t

ub.ercu.losis major secretory protein Antigen 85A
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  • ￥1080 - 87906
  • Prospecbio
  • 以色列
  • pro-1076
  • 2026年01月10日
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    上海经科化学科技有限公司
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    • 文献和实验
    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      Ag85A

    • 库存：

      常规产品有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      2ug/10ug/1mg

    规格：2ug产品价格：￥1080.0
    规格：10ug产品价格：￥2415.0
    规格：1mg产品价格：￥87906.0

    产品细节图片1

    CATALOGUE NUMBER

    PRO-1076

    SYNONYMS

    Ronectin-binding protein A, Mycolyl transferase 85A.

    INTRODUCTION

    Antigen 85A is a member of the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex have mycolyltransferase activity and catalyze the synthesis of the very rich glycolipid of the mycobacterial cell wall, the cord factor (trehalose 6,6'-dimycolate, TDM). The cord factor is vital for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A.

    DESCRIPTION

    Recombinant M.ycoba.cteriu.m tub.ercu.losis Ag85A (43-338 a.a) produced in Hi-5 cells is a single, glycosylated polypeptide chain having a molecular mass of 32.8kDa (305 a.a in total).
    Antigen 85A is fused to a 6 amino acid His tag at C-terminus and purified by conventional chromatographic techniques.

    SOURCE

    Baculovirus

    PHYSICAL APPEARANCE

    Sterile Filtered colorless solution.

    FORMULATION

    The Ag85A solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.

    STABILITY

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Avoid multiple freeze-thaw cycles.

    PURITY

    Greater than 95% as determined by SDS-PAGE.

    AMINO ACID SEQUENCE

    ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    Recombinant M.ycoba.cteriu.m tub.ercu.losis Ag85A (43-338 a.a) produced in Hi-5 cells is a single, glycosylated polypeptide chain having a molecular mass of 32.8kDa (305 a.a in total).
    Antigen 85A is fused to a 6 amino acid His tag at C-terminus and purified by conventional chromatographic techniques.

    相关实验

    • DETECTION OF RECOMBINANT PROTEINS BY ELISA

      of interest. If the recombinant protein is not soluble in H2 O, dissolve the insoluble pellet in a volume of 7 M urea equal to 1/10 of the bacterial culture volume. Vortex the samples at a medium speed for about 30 minutes (use a vortex setting of 4.5 on the Fisher Vortex Genie

    • Overview of Protein Microarrays

      to influenza A H1N1 virus, West Nile virus, and dengue virus. Cell 139:1243‐1254.    Brichta, J., Hnilova, M., and Viskovic, T. 2005. Generation of hapten‐specific recombinant

    • Strategies to Optimize Protein Expression in E. coli

      . 5:478‐487.    Baneyx, F. and Mujacic, M. 2004. Recombinant protein folding and misfolding in Escherichia coli. Nat. Biotechnol. 22:1399‐1408

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    文献支持
    Recombinant M.ycoba.cteriu.m tub.ercu.losis major secretory protein Antigen 85A
    ￥1080 - 87906