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文献和实验Influenza A Virus Molecular Virology Techniques
influenza A viruses by plasmid-based reverse genetics. To accelerate the amplification and cloning process, we developed multi-segment-RTPCR (M-RTPCR) techniques that efficiently amplify the eight genomic viral RNA segments (vRNAs) of influenza A virus
An Immunoproteomics Approach to Screen the Antigenicity of the Influenza Virus
The structure and antigenicity of protein antigens of the influenza virus are screened in a single step employing an immunoproteomics approach. Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) coupled to gel
PCR for the Detection of Influenza Viruses in Clinical Material
Influenza viruses are segmented, negative-stranded RNA viruses belonging to the family Orthomyxoviridae . They are classified into influenza A, B, and C on the basis of different epitopes on the nucleoprotein (NP) and matrix proteins (M
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