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- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
Lysostaphin
- 库存：
常规产品有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
1mg/5mg/10mg
| 规格： | 1mg | 产品价格： | ￥1150.0 |
|---|---|---|---|
| 规格： | 5mg | 产品价格： | ￥4200.0 |
| 规格： | 10mg | 产品价格： | ￥7600.0 |
CATALOGUE NUMBER
ENZ-269
INTRODUCTION
Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination.
SYNONYMS
DESCRIPTION
Lysostaphin Recombinant produced in E.Coli is a non-glycosylated polypeptide chain having a molecular mass of 26.92 kDa.
SOURCE
PHYSICAL APPEARANCE
FORMULATION
STABILITY
Lyophilized Lysostaphin although stable at room temperature for 2 weeks, should be stored desiccated below -18°C. Upon reconstitution Lysostaphin can be stored at 4°C up to 3 weeks and for future use below -18°C.
Please prevent freeze-thaw cycles.
SOLUBILITY
It is recommended to reconstitute the lyophilized Lysostaphin in 20mM sodium acetate, pH 4.5 for optimal stability, which can then be further diluted.
PURITY
98% as determined by RP-HPLC.
BIOLOGICAL ACTIVITY
Assessed by the decrease in turbidity of a suspension of heat-killed Sta.phy.loco.ccus aur.eu.s at pH-8, 30°C. Lysostaphin is a zinc enzyme therefore EDTA is an inhibitory factor.
SPECIFIC ACTIVITY
Determined to be 4,543 units/mg.
PROTEIN CONTENT
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 2.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis RP-HPLC, using a calibrated solution of Lysostaphin as a Reference standard.
SAFETY DATA SHEET
SDS
USAGE
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文献和实验Lysostaphin Recombinant produced in E.Coli is a non-glycosylated polypeptide chain having a molecular mass of 26.92 kDa.
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
enzymes; these linearize molecules can self-ligate ), the antibiotic resistance gene will allow bacteria carrying empty vector plasmids to survive the selection. Transformation The reaction used to create recombinant DNA molecules (ligation
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
-ligate ), the antibiotic resistance gene will allow bacteria carrying empty vector plasmids to survive the selection.Transformation The reaction used to create recombinant DNA molecules (ligation reaction, etc.) is used to transform bacteria
Baculovindae is a family of occluded, invertebrate-specific pathogens, consisting of two generathe nucleopolyhedroviruses (NPVs) and granuloviruses. The majority of basic and applied research efforts, as well as commercial endeavors
技术资料