Recombinant Rat Interleukin-2

T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.

IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

Interleukin-2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 16kDa.

The IL-2 is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile filtered colorless solution.

The IL-2 solution contains 0.2µm filtered solution in 20mM PB pH3.5 and 30% Glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks.

Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Avoid multiple freeze-thaw cycles.

Greater than 97.0% as determined by SDS-PAGE and HPLC.

APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ.

The ED₅₀ as determined by the dose-dependant stimulation of murine CTLL-2 cells is less than 0.2ng/ml, corresponding to a Specific Activity of 5,000,000IU/mg.