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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 组织来源:
细胞系
- 物种来源:
ATCC/sciencell/上海细胞库/自建细胞库
- 运输方式:
顺丰快递
- 年限:
第三代
- 生长状态:
贴壁/悬浮
- 规格:
1*106/T25瓶
| Synonyms: | L-363(人多发性骨髓瘤细胞) |
| Background: | established from the peripheral blood of a 36-year-old woman with plasma cell leukemia (IgG) in 1977; cells were described in the literature to be EBNA-negative and to express mRNA for proto-oncogene BCL2; plasma cell leukemia is related to multiple myeloma |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | plasma cell leukemia |
| Gender: | N/A |
| Morphology: | single, large, round or oval cells in suspension; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 25 hours |
| DNA Profile: | N/A Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+10%FBS We strongly suggest to purchase the complete medium . |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, LDH, PEP B |
| Cytogenetics: | human hyperdiploid karyotype with 11% polyploidy - 49(45-50)<2n>X, -X, -X, -6, +7, +8, +11, +11, +14, +19, -22, t(5;8)(q12/13;q24.32), der(8)(t(5;8)(q12/13;q24.32), der(8)t(7;8)(q11.2;p22), del(11)(q13)x2, add(14)(q32), del(17)(p12), add(19)(q13) - sideline with add(13)(q34) - 11q13 and 14q32 breakpoints recurrent in PCL - resembles published karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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文献和实验左旋茶氨酸 L-theanine 即谷酰基乙胺。是从茶叶中发现的( Y. Saka-to, 1950)。与谷氨酸同样机制合成。
L-DOPA (L-3,4-Dihydroxyphenylalanine)
The nonprotein amino acid, 3,4-dihydroxyphenylalanine (L -DOPA) (Fig. 1) is present in high concentrations (about 3–6%) in Mucuna spp. The in vitro addition of L -DOPA to the red blood cells from individuals deficient in glucose 6-phosphate
L-Mimosine (-(3-Hydroxy-4-Pyridone-1-yl)-L-Alanine)
L -mimosine is a nonprotein amino acid. Despite the consideration of Leucaena leucocephala as a promising alternate source of protein for fodder, the presence of mimosine to the extent of 2% to 10% dry matter in the leaf and 2% to 5% dry
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