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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human ENaC Beta. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX108842
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
ENaC Beta
- 抗体英文名:
ENaC Beta antibody [N2C1], Internal
- 抗体名:
ENaC Beta 抗体 [N2C1], Internal
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX108842 diluted at 1:1000
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文献和实验,后来发现与我买的beta-catenin一抗(中杉金桥分装的santa cruz的产品)说明书上的结果一致。请问大家做beta-catenin免疫荧光用的是哪里的抗体啊,或者是我的方法有问题吗?我也用0.5%TritonX-100打孔了,仍不行。我在这里先谢谢各位了! zhao22840718 还有一种方法不知有没有人试过,就是把beta-catenin构建到pEGFP-N3类载体中,表达融合蛋白,把载体转入细胞,然后加入你的药物。荧光显微镜下观察进核情况
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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