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- 文献和实验
- 技术资料
- 亚型:
Mouse IgG1, κ
- 形态:
液体
- 保存条件:
This product can be stored at 2-8°C for 12 months. Do not freeze.
- 克隆性:
Monoclonal
- 标记物:
None (Purified antibody-Azide Free/Low endotoxin)
- 适应物种:
Human
- 保质期:
12个月
- 目录编号:
E-AB-F12110
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 宿主:
Mouse
- 应用范围:
FCM;FA
- 抗体英文名:
AF/LE Purified Anti-Human CD11a Antibody[R7-1]_货号:E-AB-F12110
- 抗体名:
AF/LE Purified Anti-Human CD11a Antibody流式抗体[R7-1]_货号:E-AB-F12110
- 规格:
500μg/50μg/1mg
| 规格: | 500μg | 产品价格: | ¥3160.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥760.0 |
| 规格: | 1mg | 产品价格: | ¥4760.0 |

AF/LE Purified Anti-Human CD11a 抗体流式抗体[R7-1]产品名称:AF/LE Purified Anti-Human CD11a Antibody[R7-1], 货号:E-AB-F12110
| 产品描述: | CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). |
| Alternate Names | Integrin alpha-L;LFA-1A;CD11A;ITGAL; |
| Swissprot | P20701 |
| Gene ID | 3683 |
| Clone No | R7-1 |
| Host | Mouse |
| Reactivity | Human |
| Application | FCM;FA |
| 同种型 | Mouse IgG1, κ |
| 同种型 Control | AF/LE Purified Mouse IgG1, κ 同种型 Control[MOPC-21] |
| Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
| 储存方法 缓冲 | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
| 储存方法 | This product can be stored at 2-8°C for 12 months. Do not freeze. |
| Expiration date | 12个月 |
| Shipping | 冰袋 |

AF/LE Purified Anti-Human CD11a 抗体流式抗体[R7-1]labscience®拥有先进的单抗,多抗和流式抗体开发技术平台,提供8500+高品质 抗体, 适用于胞内和胞外蛋白的鉴定、定量和定位研究。抗体已在多种应用中得到验证,包含 流式细胞术(Flow Cytometry)、 免疫印迹(WB)、 免疫组化(IHC/ICC)、 免疫(共)沉淀(IP/Co-IP)、 免疫荧光(IF) 等实验。抗体产品涉及多种生命科学研究领域,包括肿瘤、免疫、心血管、细胞生物学、神经科学、表观遗传学等。Elabscience®始终致力于提高抗体的质量和种类,以保障并加快您的研究进程
This product can be stored at 2-8°C for 12 months. Do not freeze.AF/LE Purified Anti-Human CD11a 抗体流式抗体[R7-1]产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验、ionomycin和Brefeldin A,其中前两者协同激活细胞,后者抑制蛋白转运,从而有效增强胞内细胞因子的积累与检测信号。 03 封闭剂 单核细胞、巨噬细胞和B细胞表面高表达Fc受体,易非特异性结合抗体的Fc段,导致假阳性信号。为解决此问题,菲恩生物提供Purified Anti-Mouse CD16/32 Antibody(FNab30106)用于小鼠样本封闭,;Human Fc Receptor Blocking reagent(K128)用于人样本封闭。关键在于必须选择与样本
). They were isolated by gradient density centrifugation using Lymphoprep (Nycomed, Oslo, Norway). CD19+ B lymphocytes were purified by positive selection using anti-CD19-conjugated magnetic beads and a VarioMacs magnetic cell sorter (Miltenyi Biotec, Bergisch Gladbach
Elabscience|巨噬细胞极化总失败?RAW264.7/THP-1/BMDM/hMDM四大模型避坑指南与实操全攻略
检测。 M2极化:弃去旧培养基,换为无血清培养基饥饿处理16-20 h;再更换为新鲜全培养基静息培养24 h;最后加入IL-4和IL-13(50-100 ng/mL)诱导培养24-48 h,完成M2极化。 流式检测: (1)收集细胞悬液,150 × g离心3 min; (2)弃上清,加入Cell Staining Buffer或PBS缓冲液重悬细胞沉淀,调整细胞密度为1-10×106/mL; (3)取100 μL细胞悬液,每组加入2 μL Purified Anti-Mouse CD
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