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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
APC Anti-Mouse CD3ε Antibody流式抗体[145-2C11]_货号:E-AB-F1103E
- 抗体英文名:
APC Anti-Mouse CD3ε Antibody[145-2C11]_货号:E-AB-F1103E
- 应用范围:
FCM
- 宿主:
Armenian Hamster
- 适应物种:
Mouse
- 保质期:
12个月
- 目录编号:
E-AB-F1103E
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 标记物:
APC
- 克隆性:
Monoclonal
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 形态:
液体
- 亚型:
Armenian Hamster IgG
- 规格:
100Tests/50Tests/100Tests×2
| 规格: | 100Tests | 产品价格: | ¥828.0 |
|---|---|---|---|
| 规格: | 50Tests | 产品价格: | ¥480.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥1500.0 |
货号:E-AB-F1103E
| Background |
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
|
| Alternate Names |
T-cell surface glycoprotein CD3 epsilon chain;CD3E;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E;
|
| Swissprot |
P22646
|
| Gene ID |
12501
|
| Clone No |
145-2C11
|
| Host |
Armenian Hamster
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Armenian Hamster IgG
|
| Isotype Control |
APC Armenian Hamster IgG Isotype Control[PIP]
|
| Conjugation |
APC
|
| Conjugation Information |
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献详情查看>>>>


T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
1 ml 2.4 中准备好的 Biotin Elution Buffer,让液流完全进入胶体后孵育 2 min,加入 9 ml 的 Biotin Elution Buffer; 1.13 对于从全血中分选目的细胞,再加入 10 ml 的 Biotin Elution Buffer; 1.14 300 g 转速离心 10 分钟,收集洗脱目的细胞。 流程图: 2. 实验数据 以下流式图为从 7.5 ml 全血中,选出 CD3 + T 细胞的数据。选后细胞以 CD3 -APC (OKT
7211011 小鼠淋巴细胞分离液 达优 100339 Ultra-LEAF™ Purified anti-mouse CD3ε Antibody BioLegend 102115 Ultra-LEAF™ Purified anti-mouse CD28 Antibody BioLegend CD3/CD28 Streptamer® 多聚体法: 货号 名称
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