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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Purified Anti-Mouse/Human CD11b Antibody流式抗体[M1/70]_货号:E-AB-F1081A
- 抗体英文名:
Purified Anti-Mouse/Human CD11b Antibody[M1/70]_货号:E-AB-F1081A
- 应用范围:
FCM
- 宿主:
Rat
- 适应物种:
Human;Mouse
- 保质期:
12个月
- 目录编号:
E-AB-F1081A
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
This product can be stored at 2-8°C for 12 months. Do not freeze.
- 形态:
液体
- 亚型:
Rat IgG2b, κ
- 规格:
100μg/25μg
| 规格: | 100μg | 产品价格: | ¥416.0 |
|---|---|---|---|
| 规格: | 25μg | 产品价格: | ¥128.0 |
货号:E-AB-F1081A
| Background |
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.
|
| Alternate Names |
Integrin alpha-M;Itgam;CD11 antigen-like family member B;CR-3 alpha chain;Leukocyte adhesion receptor MO1;CD11b;
|
| Swissprot |
P05555;P11215;
|
| Gene ID |
16409,3684
|
| Clone No |
M1/70
|
| Host |
Rat
|
| Reactivity |
Human;Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
Purified Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
Unconjugated
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
although other techniques can be used. The choice of immobilized antibody binding protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b . Protein G binds
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
Phycoerythrin conjugation protocol
Phycoerythrin conjugation protocol David's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. Reagents Ab Antibody
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